Taking the straw biochar(S)and microbial agents(MA)as the test material,the effects of SM synergistic treatment on soil properties,enzyme activities and microbial biomass were studied.Meantime,the removal of Pb,Cd,MRGs,antibiotic and ARGs were detected by titration,chloroform fumigation-extraction and PCR.Through the above researches,the foundation of realizing waste treatment and optimizing resources would been laid.The results showd that compared with CK,the TOC,TN,TP and AP in soil were significantly improved with SM.The activities of catalase,urease and invertase in soil with SM were increased by 36.00%,45.00%and 53.00%,respectively.MBC,MBN and MBP in siol were also increased.In Cd-Pb-SM and Cd-Pb-T-SM groups,the residual of Cd and Pb were reduced to 35.49%and 20.09%,respectively.While the repair rates of Cd and Pb were 23.69%and 25.57%in Cd-Pb-T-SM and Cd-Pb-T group,respectively.Compared with Cd-Pb group,the cad D and pbrT of Cd-Pb-SM group were decreased by 31.94%and 30.11%,respectively.While those of Cd-Pb-T-SM group were decreased by 29.19%and 35.74%,respectively.Compared T,Cd-T,Pb-T,Pb-Cd-T groups with T-SM,Cd-T-SM,Pb-T-SM,Pb-Cd-T-SM,Pb-Cd-T-SM groups,the residues of tetracycline were increased towere 23.83%,24.03%,10.85%,19.08%,respectively.Compared Cd-Pb-T-SM with Cd-Pb-T-SM group,tet A and tet M were decreased to 30.32%and 31.71%,respectively.In conclusion,all results had showed that the straw biochar combined with microbial agent could effectively improved soil structure and circulation,promoted soil enzyme activity.Meamtime,these also had been effected on multi-pollution of heavy metals and antibiotics.