Adaptation of Seed Germination and Seedling Growth of Brassica oleracea L.var.capitata L.to Different Concentrations of Cd
Taking seeds of Brassica oleracea L.var.capitata L.as the test material under different levels of Cd stress(0,25,50,100,200 mg·L-1 and 400 mg·L-1).The effects of different Cd concentrations on seed germination characteristics,seedling shoot and root elongation,biomass,chlorophyll content,leaf malondialdehyde content,leaf relative conductivity,leaf osmoregulatory substances content(soluble sugars and soluble proteins),and root vigor of Brassica oleracea L.var.capitata L.were investigated in a potting contamination simulation experiment,in order to provide reference for the breeding of Brassica oleracea L.var.capitata L.and identification of plant damage due to soil Cd contamination.The results showed that,1)high concentration(＞100 mg·L-1)of Cd stress significantly reduced the germination rate,germination potential,germination index and vigor index of Brassica oleracea L.var.capitata L.seeds,while low concentration(＜100 mg·L-1)of Cd stress did not significantly affect germination potential and germination rate,but resulted in a decrease in germination index and vigor index.2)Different concentrations of Cd stress inhibited shoot and root elongation,root vigor,and chlorophyll content accumulation in Brassica oleracea L.var.capitata L.seedlings,while biomass,soluble sugar,and soluble protein content showed inhibition only under Cd stress higher than 100 mg·L-1.In addition,Cd stress higher than 100 mg·L-1 also led to a significant increase in the relative conductivity of leaf blades of Brassica oleracea L.var.capitata L.seedlings.However,none of the different concentrations of Cd stress significantly affected malondialdehyde content and root-crown ratio.In summary,seed germination and seedling growth of Brassica oleracea L.var.capitata L.were tolerant to Cd stress below 100 mg·L-1,while Cd concentration higher than 100 mg·L-1 inhibited seed germination and seedling growth of Brassica oleracea L.var.capitata L.to a greater extent.
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