Analysis of Saline-alkali Stress on Physiological Response of Leptodermis oblonga Bunge.Seedlings
The tissue culture plantlets of Leptodermis oblonga were used as the test materials,and different concentrations(50,100,150,200,250 mmol·L-1)of saline-alkali stress were used,and the no saline-alkali solution was used as the control,malondialdehyde(MDA)content,peroxidase(POD)activity,catalase(CAT)activity and chlorophyll content in the leaves of Leptodermis oblonga were determined to study the effects of saline-alkali stress on physiological characteristics of Leptodermis oblonga seedlings,the salt-alkali tolerance of the seedlings was determined,in order to provide reference for its introduction and cultivation.The results showed that MDA content increased with the increase of saline-alkali stress concentration and the extension of stress time,with the increase of saline-alkali stress concentration and the extension of stress time,the activity of CAT increased first and then decreased.With the increase of salt stress concentration and the extension of stress time,the POD activity increased first and then decreased,and the peak value of salt stress was 50 mmol·L-1,the change of POD activity was not consistent under alkali stress.With the increase of salt stress concentration and the extension of stress time,the content of chlorophyll decreased,there was no significant difference between 50 mmol·L-1,100 mmol·L-1 and the control under alkali stress,which indicated that Leptodermis oblonga seedlings had a certain ability of salt tolerance.At a salinity concentration of 50 mmol·L-1,the plant adaptation to the environment could be protected by its own physiological and biochemical changes.When the salinity concentration was greater than 150 mmol·L-1,the seedlings of Leptodermis oblonga were damaged by salinity stress,and affected the normal process of plant growth.
Leptodermis oblonga Bunge.seedlingssaline-alkali stressphysiological and biochemical index