Response Surface Optimization of Ultrasound-assisted Ethanol Extraction of Shikimic Acid From Ginkgo biloba Leaves
Shikimic acid was extracted from dried leaves of Ginkgo biloba L.by ultrasonic-assisted 70％ethanol.The extraction process was optimized by single factor experiment and response surface experiment.The effects of material-liquid ratio,extraction temperature,extraction time and extraction times on the extraction rate of shikimic acid were studied,and the optimum extraction process was optimized,in order to provide reference for the extraction of shikimic acid from Ginkgo biloba leaves.The results showed that the optimum technological conditions were as follows,material-liquid ratio 1︰8 g·mL-1,extraction temperature 66 ℃,extraction time 65 minutes(once for each extraction)and extraction times twice.Under these conditions,the extraction yield of shikimic acid from Ginkgo biloba leaves was 1.805％,which was close to the theoretical yield(1.883％).
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