Study on Tissue Culture Rapid Propagation and Tissue Micro-cuttage Technology of Pyrus betulifolia
Tissue culture plantlets of 4 Pyrus betulifolia lines originated from seed were used as the test materials.The methods of plant tissue culture and tissue culture seedling micro-cutting were adopted.The effects of different concentrations of 6-BA singly or in combination with IBA on the in vitro shoot proliferation and the influence of different concentrations of IBA or NAA on rooting of micro-shoots by in vitro and ex vitro was studied,in order to provide reference for the establishment of the tissue culture and rapid proliferation system of Pyrus betuli folia.The results showed that the genotype and 6-BA concentration had a highly significant effect on the proliferation coefficient,and the IBA concentration had a significant effect on the proliferation coefficient.As for root induction,low concentration of auxin was better than high concentration.DL30 showed higher proliferation coefficient and rooting rate.The optimum medium for DL30 shoot proliferation was MS+1.0 mg·L-16-BA+0.2 mg·L-1 IBA,with a proliferation coefficient 3.4±0.35.For the in vitro rooting experiments,the highest rooting rate of DL30 was 60.0％±20.0％on the medium containing 1/2MS+0.2 mg·L-1 IBA.For the ex vitro rooting experiments,rooting of micro-shoots was significantly influenced by auxin applied as quick-dip method,and the highest rooting rate(46.7％±8.8％)was obtained in DL30 at the treatment of 1 000 mg·L-1 IBA.
万方数据
维普
