Taking two Lycium barbarum varieties,'Ningqi No.7'(V1)and'Xuedizi'(V2),as test materials,pot experiments were conducted to establish four treatment groups with Bacillus subtilis,a control group(CK),and three treatment groups T1(3 g·L-1),T2(6 g·L-1),and T3(9 g·L-1).The effects of Bacillus subtilis on the quality components and soil characteristics of different Lycium barbarum varieties were studied,in order to provide reference for cultivation and quality evaluation of different varieties of Lycium barbarum.The results showed that the fruit shape indexes of V1 were superior to those of V2 under all treatments.The content of betaine in T2 treatment was 2.29%-19.77%(V1 variety)and 7.90%-30.60%(V2 variety)higher than that in CK,T1 and T3 treatment.The fruit form index,soluble solids content,lycium polysaccharide,betaine and flavonoid contents of V2 variety were higher than those of V1 variety.T2 treatment was the best,but the effects of each treatment on soil characteristics were not significant.