Anoectochilus roxburghii resources'Meihuashan'treated at 35 ℃ for 3 days and 4 days were used as the test materials.Principal component analysis(PCA)was used to study the effects of high temperature acclimation on plant traits,physiological and biochemical indexes and nutritional quality of Anoectochilus roxburghii,in order to understand the thermal sensitivity of Anoectochilus roxburghii'Meihuashan'to temperature,and to provide reference for the screening of new heat-resistant strains or new varieties and the improvement of cultivation techniques.The results showed that high temperature acclimation reduced the sprouting rate and the plant height,increased the stem diameter and dry matter content.High temperature acclimation could increase the chlorophyll content and the soluble protein contentdecrease the relative conductivity of Anoectochilus roxburghii.High temperature acclimation would reduce the content of reducing sugar,flavonoids and total free amino acids in Anoectochilus roxburghii,increase the content of polysaccharides and total phenols,and increase or decrease the content of trace elements in Anoectochilus roxburghii.The germination rate,plant height,stem diameter,weight per plant,dry matter content,chlorophyll content,relative conductivity,soluble protein,reducing sugar,polysaccharide,flavonoids,total phenols,total free amino acids,Cu,Zn,Ca,Mg,Fe,Mn,K and other 20 elements were analyzed by PCA.A total of 2 principal components were identified,and the cumulative variance contribution rate was 100.00％.The results of principal component analysis showed that the heat resistance of Anoectochilus roxburghii was highest after 4 days of high temperature acclimation at 35 ℃,followed by 3 days treatment.High temperature acclimation is an effective measure to improve the heat resistance of Anoectochilus roxburghii.
high temperature acclimationAnoectochilus roxburghiiphysiological indicatorsnutritional content
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