北方园艺2025,Issue(1) :8-14.DOI:10.11937/bfyy.20242577

基于SRAP分子标记的五十份木槿种质资源亲缘关系的分析

Analysis the Phylogenetic Relationship of 50 Germplasm Resources of Hibiscus syriacus L.Based on SRAP Molecular Markers

方茹 罗优波 胡亚芬 王衍彬 王丽玲 杨少宗
北方园艺2025,Issue(1) :8-14.DOI:10.11937/bfyy.20242577

基于SRAP分子标记的五十份木槿种质资源亲缘关系的分析

Analysis the Phylogenetic Relationship of 50 Germplasm Resources of Hibiscus syriacus L.Based on SRAP Molecular Markers

方茹 1罗优波 2胡亚芬 2王衍彬 1王丽玲 1杨少宗1
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作者信息

  • 1. 浙江省林业科学研究院,森林食品资源利用与质量控制国家林业局重点实验室,浙江 杭州 310023
  • 2. 杭州市林业和湿地科学研究院,浙江 杭州 310022
  • 折叠

摘要

以木槿(Hibiscus syriacus L.)为试材,采用UPGMA法,通过16对SRAP引物组合研究50份木槿种质亲缘关系,以期为加速木槿新品种的选育进程提供参考依据.结果表明:16对SRAP引物组合共扩增出390条条带,其中有388条具有多态性,多态性比率平均为99.49%.在遗传距离为0.362 0处,50份木槿样本被分为5组,分类结果显示木槿品种间的亲缘关系与木槿花花瓣基部色斑和花瓣型这2个形态特征呈现密切相关,而与花色以及植株、叶片以及果实等形态特征未呈现相关性.综上所述,SRAP标记技术对木槿基因组检测多态性水平高,可用于木槿种质资源鉴定以及分子标记辅助育种等工作.

Abstract

Taking Hibiscus syriacus L.as the test material,UPGMA method was adopted,the genetic relationships of 50 Hibiscus syriacus L.were analyzed by 16 pairs of SRAP primers,in order to provide reference for accelerating the breeding process of new Hibiscus syriacus L.varieties.The results showed that 16 SRAP combining primers produced 390 bands,included 388 polymorphic bands,with a polymorphism ratio of 99.49%.At the genetic distance of 0.362 0,50 Hibiscus syriacus L.varieties were classified into 5 categories.The results of clustering analysis revealed significant correlation among phylogenetic relationship of Hibiscus syriacus L.varieties and two morphological characteristics of flower,basal blotches in the petal and petal-type.And yet,there was no significant correlation between phylogenetic relationship and flower color.In addition,there was no significant correlation between phylogenetic relationship and the morphological characteristics of plant,leaf and fruit.In conclusion,the SRAP markers,showing high level of polymorphism in the genome detection of Hibiscus syriacus L.,could be used as an effective means for identification of the germplasm resources and molecular marker-assisted breeding of Hibiscus syriacus L.

关键词

木槿/SRAP分子标记/种质资源/亲缘关系

Key words

Hibiscus syriacus L./SRAP molecular markers/germplasm resources/phylogenetic rela-tionship

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出版年

2025
北方园艺
黑龙江省农业科学院 黑龙江省园艺学会 黑龙江省农业科学院编辑出版中心

北方园艺

北大核心
影响因子:0.506
ISSN:1001-0009
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