Identification and Biological Characteristics Analysis of the Pathogen Causing the Sclerotium Disease of Fritillaria ussuriensis Maxim.
The sclerotium diseased plants of Fritillaria ussuriensis Maxim,in Tieli city were used as test materials by using tissue isolation,morphological observation and molecular biology analysis.The taxonomic status and biological characteristics of the pathogen were studied with the expectation of clarifying the pathogen of the sclerotium disease of F.ussuriensis Maxim.in Tieli city and growth characteristics of the pathogen,in order to provide reference for better developing prevention and control measures.The results showed that the two pathogenic strains PBJH-1 and PBJH-2 causing the sclerotium diseasing of F.ussuriensis Maxim.in Tieli city,were Sclerotium denigrans.The optimal growth temperature of the two pathogenic strains was 20 ℃,and they stopped growing at 30 ℃.There was no significant difference in the growth rate under different light conditions.The optimal pH for mycelia growth was 4.0,and the optimal carbon source was malt sugar.The best nitrogen source of PBJH-1 was peptone and PBJH-2 was yeast extract.
万方数据
维普
