Expression Changes of AMPK-alpha in Skeletal Muscle Fatty Acid Metabolism and Insulin Resistance in Patients with Diabetes Mellitus
ObjectiveTo investigate the role of the expression changes of adenosine monophosphate activated protein kinase alpha (AMPK-α) in skeletal muscle fatty acid metabolism and insulin resistance in patients with diabetes mellitus.Method20 patients with type 2 diabetes for 5 or less than 5 years from the patients who had undergone hip and lower limb surgery were selected as study group,and 20 non-diabetic patients who had undergone the orthopedic operations were selected as control group.The patients fasting venous blood samples were taken for the detection of biochemical indexes,the skeletal muscle tissue was left during the orthopedic surgeries for the determination of TG and long chain fatty acyl coenzyme A (LCACoA) levels in the skeletal muscle.RT-PCR method was used to detect the level of AMPK-α1 and AMPK-α2 mRNA in the skeletal muscle;Western blotting was used to detect the level of AMPK-α1 and AMPK-α2,and phosphorylated AMPK-α protein in the skeletal muscle.ResultsFasting blood glucose,glycosylated hemoglobin,insulin,TG and FFA levels were significantly higher than those in the control group,ISI was significantly lower than that in the control group,and the difference was statistically significant (P<0.05);TG and LCACoA in the skeletal muscle in the study group were obviously higher than those in the control group,with statistically significant differences (P<0.05).There was no significant difference in the number of AMPK-α1 mRNA copy between the study group and the control group (P>0.05);the AMPK-α1 mRNA copy number in the study group was significantly lower than that in the control group,showing a statistically significant difference (P<0.05).The difference in AMPK-α1 protein expression was not statistically significant between the study group and the control group (P>0.05);the AMPK-alpha 2 and phosphorylated AMPK-α protein levels in the study group were significantly lower than those in the control group (P<0.05).ConclusionThe abnormal fatty acid metabolism and insulin resistance are common in patients with type 2 diabetes mellitus,the expression of AMPK-α2 and the changes of AMPK-α activity play a key role in the lipid accumulation and insulin resistance in skeletal muscle.
type 2 diabetes mellitusskeletal muscleinsulin resistanceAMPK-α
国家科技期刊平台
NSTL
万方数据
维普
