Effects of Different Shading Conditions on Photosynthetic Characteristics and Ultrastructure of Ilex verticillata Seedling Leaves
To study the photosynthetic physiological characteristics and ultrastructure of Ilex verticillata seedlings under different shading conditions,aiming to reveal their adaptation to light demand,growth characteristics in the Northeast region and photosynthetic and ultrastructure relationship,and provide a basis for the introduction,cultivation and management of Ilex verticillata seedlings.The photosynthetic target and chlorophyll fluorescence of 3-year-old Ilex verticillata seedlings were measured under four shading conditions:full light(L0),35%shading(L35),55%shading(L55),and 75%shading(L75)using Li-6800 photosynthetic apparatus.The leaf ultra-structure of Ilex verticillata was observed by using scanning electron microscopy.The daily variation curve of net photosynthetic rate in the four treatment groups in July showed a single peak;The light response curve of Ilex verticillata seedlings showed the best performance in the 35%shading.As the degree of shading increases,Fo,F′m,F′v,F′v/F′m,FS,and qP under light adaptation gradually increase;The QNP of Ilex verticillata seedlings decreases with increasing shading,and the leaves of Ilex verticillata alleviate photosynthetic damage by consuming heat dissipation energy under strong light stress,exhibiting high light protection ability.The stomatal opening of leaves in Ilex verticillata seedlings increases with increasing light intensity,but excessive light can inhibit stomatal opening.When the net photosynthetic rate is at its maximum,the stomatal opening degree is at its maximum,and the size and opening degree of stomata will change with the change of net photosynthetic rate.