Effect and Mechanism of Cucurbitacin B on Improving Lipopolysaccharide-induced C2C12 Myotube Atrophy
Objective To explore the influence of Cucurbitacin B(CuB)on LPS-induced C2C12 myotube atro-phy and skeletal muscle atrophy in C57BL/6 mice.Methods Protein expression levels were determined by Western blot,cell morphological alterations were observed using H&E staining,and the degree of skeletal muscle atrophy was evaluated by measuring the grip strength of mice and the mass of gastrocnemius and tibialis anterior muscles.Results Western blot experiment indicated that LPS was capable of inducing the atrophy of C2C12 myotubes(P<0.01),while treatment with CuB(0.3～3.0 μmol/L)could promote the expression of myotube differentiation-related proteins(MyHC)and muscle synthesis-related proteins,and reduce the release of proinfla-mmatory factors(IL-1β,COX-2)(P<0.001).The results of H&E staining assay confirmed morphologically that CuB could effectively ameliorate the myotube atrophy stimulated by LPS.Additionally,the animal experiments revealed that LPS could lead to a significant decline in the grip strength of C57BL/6 mice and the mass of gastrocnemius and tibialis anterior muscles,and the symptoms were improved after treatment with CuB(P<0.05).Conclusion CuB holds potential application value in treatment of skeletal muscle atrophy.It offers a theoretical foundation for the development of lead compounds from natural medicinal sources and pioneers a novel thinking for the treatment of inflammatory skeletal muscle atrophy.
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