Protective Effect of Curdione on Acute Liver Injury Induced by APAP
Objective To investigate the protective effect of curdione(CUR)on acute liver injury induced by acetaminophen(APAP).Methods 6～8-week-old male C57BL/6 mice were randomly divided into control group(CON),APAP model group(APAP),CUR low-dose administration group(25 mg/kg)and CUR high-dose administration group(50 mg/kg),with six mice in each group.After three consecutive days of gavage,APAP(400 mg/kg)was injected intraperitoneally into each group except the CON group to establish a liver injury model.Six hours after APAP injection,the blood and liver samples of mice were taken to detect the levels of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum.Hematoxylin and eosin(H&E)were used to stain liver tissue sections to assess liver damage.The expression levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in liver tissues were measured.Additionally,the protein expression levels of apoptotic factors(Bax,Bcl-2,Cleaved Caspase 3)and oxidative stress factors(Nrf2,HO-1 and NQO-1)in liver tissues were detected by Western blot.Hepatocyte apoptosis was detected by TUNEL staining.Results Compared with model group,the serum levels of AST and ALT in administration group were significantly reduced(P<0.05,P<0.01).H&E staining of liver tissue confirmed that CUR could significantly alleviate the morphological and structural changes of mouse liver tissue.In administration group,the content of MDA in liver tissue was significantly decreased,while the activity of SOD was significantly increased(P<0.05,P<0.01).Western blot results showed that CUR significantly increased the protein expression of oxidative stress factors.Additionally,the protein expression of apoptosis factors Bax and Cleaved Caspase 3 was significantly down-regulated,while the protein expression of Bcl-2 was significantly up-regulated(P<0.05,P<0.01).The results of TUNEL staining showed that CUR could significantly reduce APAP-induced hepatocyte death.Conclusion Curdione can significantly improve APAP-induced acute liver injury.
国家科技期刊平台
NSTL
万方数据
