Effects of Moderate Intensity Continuous Training and High-intensity Interval Training on Autism Model Mice
Objective To explore effects of moderate intensity continuous training and high-intensity interval training on autism model mice.Methods Twenty-one healthy adult C57BL/6 mice(7 males and 14 females)were caged together and bred.After conception,14 female mice were randomly assigned to model group(maternal group)and control group(maternal group);model group(maternal)received treatment with valproic acid injection(concentration 250 mg/mL,injection volume 1.2 mL/kg)on the 10th and 12th day of pregnancy,while control group(maternal)received the same volume of 0.9%sodium chloride injection.Model group(offspring)underwent a mother child separation experiment 4 days after birth,and after 24 hours of separation,they were returned to their parents.Establish an autism model.After 12 weeks of modeling,24 mice were selected from model group(offspring),which were respectively set as model group,medium intensity continuous training group,and high-intensity interval training group.In addition,8 mice were selected from control group(offspring)for subsequent experiments.Behavioral changes in mice were observed by grooming test and socialization test;expression levels of phosphatase tension protein homologue,nuclear factor-κB(NF-κB),and inflammatory vesicle NOD-like receptor protein 1(NLRP1)were detected by Western blot;and expression of Caspase-1 in the dentate gyrus region of hippocampus was detected by immunohistochemistry.Results The number of haircuts in model group mice was significantly longer than that in control group,the number of haircuts in moderate intensity continuous training group mice was significantly shorter than that in model group,and the number of haircuts in high-intensity interval training group mice was significantly shorter than that in moderate intensity continuous training group(all P<0.05).In the social experiment,no aggressive behavior was found in any group of mice,but in terms of non aggressive behavior,the interaction time of model group mice was significantly shorter than that of control group,the interaction time of moderate intensity continuous training group mice was significantly longer than that of model group,and the interaction time of high-intensity interval training group mice was significantly longer than that of moderate intensity continuous training group mice(all P<0.05).The differences were statistically significant when comparing the phosphatase tension protein homologue,NF-κB,and NLRP1 proteins of mice in each group.The expression of phosphatase tension protein homologue,NF-κB,and NLRP1 proteins in mice in model group was significantly higher than that in control group,moderate intensity continuous training group was significantly lower than model group,and high intensity gap training group was significantly lower than that in model group and moderate intensity continuous training group(all P<0.05).The number of apoptotic cells in control group was significantly lower than that in model group(P<0.01).The number of apoptotic cells in moderate intensity continuous training group was significantly lower than that in model group,and high-intensity intermittent training group was significantly lower than that in moderate intensity continuous training group(P<0.01).Conclusion Sports training can reduce apoptosis in the dentate gyrus region of the hippocampus and improve the symptoms of autistic mice by inhibiting the activation of phosphatase tension protein homologs,NF-κB,and NLRP1,in which the effect of high-intensity interval training is more obvious.
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