Functional identification and analysis of PtNRT2 7 gene from Populus trichocarpa
In present study, we successfully isolated a gene from NRT2 family named PtNRT2. 7. Bioinformatic analyses showed that PtNRT2. 7 had 11 transmembrane regions, and hydrophobic regions and hydrophilic regions are distributed alternately on cell membrane without signal peptide. Subcellular localization analysis showed that the PtNRT2. 7-GFP fusion protein is distributed on cell membrane. According to above findings, it belongs to a membrane protein. The expression features of Populus trichocarpa showed that PtNRT2. 7 expresses in leaves, with the highest expression in mature leaves; the highest expression appears at 1 h under the nitrate stress;under the stress of SA/JA/GA/IAA, the expression of PtNRT2. 7 is up-regulated in both above-and underground parts;under the stress of Eth/ABA/NaCl, the expression of PtNRT2. 7 is down-regulated. The expression vector was constructed and transformed into Arabidopsis thaliana wild type ( Col-0 ) and atnrt2. 7 mutant using floral dip method. The stress of different concentrations of KNO3 showed that, when the concentration of KNO3 is more than 0. 1 mmol/L, the root enlongation of overexpression lines is longer than others; the mutant lines are inhibited when the concentration is more than 1 mmol/L but the complementary lines can remove the inhibition. The result showed that hormones and NO3- can induce PtNRT2. 7, enhance the root growth and promote the absorption of nitrogen.
Populus trichocarpaPtNRT2. 7sequence analysisfunctional identification
国家科技期刊平台
NSTL
万方数据
维普
