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胡杨PeMAX2调控拟南芥耐旱性

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[目的]MAX2能抑制植物的分枝，且是独角金内酯信号传导途径的关键调控因子。MAX2同时还参与多种激素间的交叉互作，在植物抵御生物和非生物胁迫中发挥至关重要的作用。前期研究发现胡杨PeMAX2能调控拟南芥的离子平衡和耐盐性，但对于PeMAX2的耐旱功能还知之甚少，本文将探索胡杨PeMAX2调控拟南芥耐旱性的作用机制。[方法]将胡杨PeMAX2在拟南芥中异源表达，进行渗透胁迫和干旱胁迫，研究过表达PeMAX2拟南芥的生理和分子响应机制。[结果](1)在长期干旱胁迫下，胡杨叶片PeMAX2基因表达水平升高。(2)甘露醇处理后，PeMAX2转基因拟南芥的种子萌发率和根长均高于野生型拟南芥(WT)和max2突变体，并且转基因株系细胞膜受损较轻。渗透胁迫后，转基因株系超氧化歧化酶、过氧化物酶、过氧化氢酶活性及其编码基因上调表达的升高幅度均显著高于拟南芥WT和max2突变体，转基因株系调控根细胞H2O2水平的能力更强。(3)土壤干旱处理10 d后，转基因株系叶绿素含量下降幅度低于WT和max2突变体，而且过表达株系在干旱胁迫下能维持较高水平的PS Ⅱ最大光化学效率、相对电子传递效率和实际光合量子产量;在碳同化和气孔导度方面，转基因拟南芥叶片的净光合速率和气孔导度均高于WT和突变体，说明过表达PeMAX2提高了拟南芥转基因植株在干旱条件下光合作用的能力。土壤复水后WT和突变体叶绿素含量、荧光和光合作用的恢复程度也明显低于转基因株系。[结论]过表达胡杨PeMAX2可以提高拟南芥的耐旱性，主要是由于过表达PeMAX2提高了转基因拟南芥活性氧平衡的调控能力，减弱了干旱胁迫对生物膜的氧化损伤和光合作用的抑制。
Populus euphratica PeMAX2 regulating drought tolerance in Arabidopsis thaliana
[Objective]MAX2 plays an important role in inhibiting plant branching and regulating strigolactone signaling pathway.MAX2 is also involved in multi-phytohormone interactions and in plant response to biotic and abiotic stresses.It has been shown that overexpression of Populus euphratica PeMAX2 can improve ionic homeostasis and the salt tolerance in transgenic plants of Arabidopsis thaliana.However,little is known about the function of PeMAX2 in drought tolerance.The objective of this study is to explore the mechanism of PeMAX2 in regulating drought tolerance of Arabidopsis thaliana.[Method]P.euphratica PeMAX2 was overexpressed in A.thaliana,and the physiological and molecular mechanism underlying the osmotic and drought tolerance of transgenic plants was investigated in this study.[Result](1)The expression of PeMAX2 gene was up-regulated in P.euphratica leaves under long-term of drought stress.(2)After mannitol treatment,the seed germination rate and root length of A.thaliana overexpressing PeMAX2 were significantly higher than those of wild type and max2 mutant.The cell membrane was less damaged in PeMAX2-transgenic plants under osmotic stress compared with WT and max2.The increase of superoxide dismutase,peroxidase and catalase activities and the transcription levels of their encoding genes were higher in transgenic lines than in WT and max2 mutant under osmotic stress.As a result,the ability to regulate H2O2 was increased in root cells of transgenic lines.(3)A 10-d of soil drought decreased the chlorophyll content in all tested lines,and a more pronounced reduction was observed in WT and max2 mutant.Under drought stress,the maximum photochemical efficiency of PS Ⅱ,relative electron transport rate and actual quantum yield of photosynthesis were less inhibited in PeMAX2-overexpressed plants than in WT and max2.Meanwhile,transgenic plants had higher net photosynthetic rate and stomatal conductance under drought treatment,as compared with WT and mutant plants.This indicated that overexpression of PeMAX2 improved the photosynthetic capacity of transgenic plants under drought conditions.The recovery of chlorophyll content,fluorescence and photosynthesis of WT and mutant were significantly lower than that of transgenic lines after soil rehydration.[Conclusion]Overexpression of P.euphratica PeMAX2 improves the drought tolerance of A.thaliana.This is mainly due to the increased ability to scavenge reactive oxygen species in transgenic plants.Consequently,the oxidative damage to cell membrane and the drought inhibition of photosynthesis are alleviated in PeMAX2-transgenic plants under water stress.

Populus euphraticaPeMAX2Arabidopsis thalianadrought toleranceantioxidant enzymephotosynthesis

马思圆、尧俊、李静、安珂悦、赵瑞、赵楠、周晓阳、陈少良

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北京林业大学生物科学与技术学院,林木、花卉遗传育种教育部重点实验室,北京 100083

广东省森林培育与保护利用重点实验室,广东省林业科学研究院,广东广州 510520

北京林业大学验室与设备管理处,北京 100083

胡杨 PeMAX2 拟南芥 耐旱性 抗氧化酶 光合作用

国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目高等学校学科创新引智计划项目高等学校学科创新引智计划项目北京市自然科学基金项目北京市自然科学基金项目中央高校基本科研业务费专项

320717303237182831770643111 ProjectB130007618203061720242019ZY25

2024

10.12171/j.1000-1522.20220494

北京林业大学学报
北京林业大学

北京林业大学学报

CSTPCD北大核心
影响因子：1.237
ISSN：1000-1522
年,卷(期)：2024.46(6)
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