Effects of Xingnaojing on Neuroinflammation in Rats with Traumatic Brain Injury by Regulating CREB/PGC-1α Signal Pathway
Objective To analyze the effect of Xingnaojing on neuroinflammation in rats with traumatic brain injury(TBI)by regulating cAMP response element binding protein(CREB)/peroxisome proliferator activated receptor γ coactivator 1α(PGC-1α)pathway.Methods 75 SD rats were divided into the control group,model group,low-dose Xingnaojing group(0.52mL/100g),high-dose Xingnaojing group(1.04mL/100g),and high-dose Xingnaojing group+666-15(CREB inhibitor)group(10mg/kg),with 15 rats in each group.Traumatic brain injury models were established in all groups except for the control group.The nerve injury severity score(NSS)of all rats was calculated,while the brain water content was measured by dry-wet method.TUNEL method was used to measure the apoptosis of nerve cells in rats.Levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in brain tissue of rats were measured by ELISA.The expression of CREB/PGC-1α pathway protein was determined by western blot.Results Compared with the control group,NSS score,brain water content,neuronal apoptosis rate,TNF-α,IL-1β,and IL-6 levels in brain tissue,p-CREB/CREB,PGC-1α protein expression in the model group increased(P＜0.05);Compared with the model group,the NSS score,brain water content,neuronal apoptosis rate,TNF-α,IL-1β and IL-6 levels in brain tissue of rats in low-dose and high-dose Xingnaojing groups decreased,while the expression of p-CREB/CREB and PGC-1α proteins in brain tissue increased(P＜0.05);Compared with the high dose Xingnaojing group,NSS score,brain water content,neuronal apoptosis rate,TNF-α,IL-1β and IL-6 levels in brain tissue of rats in the high dose Xingnaojing+666-15 group increased,while the expression of p-CREB/CREB and PGC-1αproteins in brain tissue decreased(P＜0.05).Conclusion The current study suggests that Xingnaojing may inhibit neuroinflammation in TBI rats by activating CREB/PGC-1α pathway.
XingnaojingTraumatic brain injuryNeuroinflammationCREB/PGC-1α signal pathway
NETL
NSTL
万方数据
维普
