An Analysis of IFN-γ Combined with IL-6 in the Diagnosis of Bacillus-negative Pulmonary Tuberculosis
Objective To investigate the application value of IFN-γ combined with IL-6 in the clinical diagnosis of mycobacterium-negative pulmonary tuberculosis and its clinical significance.Methods 266 cases were collected from October 21,2021 to April 27,2023 for the examination at the Infectious Diseases Hospital of Soochow University.According to the diagnostic criteria of pulmonary tuberculosis (WS288-2017 ),the following groups were identified:196 patients with pulmonary tuberculosis (130 males,66 females,mean age 58.4 ± 17.1 ),141 patients with bilateral lesions,55 patients with unilateral lesions,92 patients with bacteriophage positive,104 patients with bacteriophage negative.There were 70 patients with occupational pneumoconiosis,including 69 male and 1 female with mean age of 62.7±8.9.There were 20 cases in the healthy control group,including 10 males and 10 females with mean age of 58.6±6.3.The expression levels of cytokines (IFN-γ,IFN-α,IL-2,IL-4,IL-5,IL-6,IL-8,TNF-α,IL-10,IL-12P70,IL-1β,IL-17)in plasma were detected by flow cytometry to evaluate the differences of these twelve inflammatory cytokines in different groups.Results (1)plasma IFN-γ and IL-6 levels were significantly higher in the group of patients with active tuberculosis than in the group of healthy controls as well as in the group of patients with occupational pneumoconiosis (non-tuberculous lung disease controls);(2)In patients with active tuberculosis,plasma IL-6 and IL-8 levels were significantly higher in the group of patients with bilateral tuberculosis lesions than in the group of patients with unilateral tuberculosis lesions;(3)In patients with active tuberculosis,plasma IL-6 levels were significantly higher in the group of patients with bacillus-positive tuberculosis than in the group of patients with bacillus-negative tuberculosis;(4)In patients with active tuberculosis,the levels of IFN-γ and IL-6 were significantly higher in the group of patients with negative gamma-interferon release assays than in the group of healthy controls;(5 )In patients with mycobacterium-negative tuberculosis,IFN-γ and IL-6 levels were significantly higher in the group of patients with negative gamma-interferon release assays than in the healthy control group.Conclusion IFN-γ,IL-6,IL-8 can reflect the inflammation status,disease severity and bacterial load of tuberculosis patients,and IFN-γ combined with IL-6 can be used as an auxiliary indicator for the diagnosis of patients with no evidence of pathogenicity,negative immunological examination results and a pulmonary imaging basis.Clinicians can utilize expression levels of IFN-γ, IL-6 combined with the evidence of pulmonary imaging to carry out auxiliary diagnosis of such patients,as well as to assess the immune status of the patient to improve patients'immunity and to provide the knowledge basis of personalized treatment.
Active tuberculosisBacteria-negative tuberculosisInflammatory cytokinesIFN-γIL-6
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