Effects and Mechanisms of Lactobacillus Plantarum P9 on Improving the Immune Function of Mice
Objective To investigate the regulatory function and underlying mechanisms of Lactobacillus plantarum P9 on immune function in mice.Methods C57BL/6J mice were treated with low-,medium-or high-doses of Lactobacillus plantarum P9 (0.5mg/kg, 1.0mg/kg or 3.0mg/kg ) by gavage, respectively.The general condition of these mice was recorded daily.After 30 days,all mice were sacrificed to calculate the organ index.Serum hemolysin and antibody-producing cells were used to study the humoral immune function; Delayed-type hypersensitivity test and lymphocyte transformation test were applied to study the cellular immune function.Phagocytosis of chicken erythrocytes by peritoneal macrophages,the carbon clearance test and the activity of natural killer (NK)cell activity were studied.Results There was no significant difference in organ index among the control group,low-dose,medium-dose and high-dose of Lactobacillus plantarum P9.After treatment with Lactobacillus plantarum P9,the serum hemolysin production,delayed-type hypersensitivity, phagocytosis of chicken erythrocytes by peritoneal macrophages,carbon clearance rate and NK cell activity were all significantly increased in mice.Conclusion Lactobacillus plantarum P9 can improve the immune function of mice by regulating humoral immunity, cellular immunity, monocyte-macrophage function and NK cell activities.
Lactobacillus plantarum P9Immune functionMouseMechanism
万方数据
维普
