Optimization and Evaluation of Tail Vein Injection Technique for Micro-PET Imaging of Mouse 13N-NH3H2O Myocardial Perfusion
Objective To provide a practical,fast and successful experimental tool for 13N-NH3·H2O myocardial perfusion imaging in mice by using a homemade mouse tail vein needle and to summarize a pipeline of smooth and efficient experimental procedures.Methods The experimental group and the control group used a mouse tail vein needle and a human peripheral superficial vein single-use intravenous needle (JERRY 26G),respectively,to perform tail vein needle puncture in the mice, with the dynamic acquisition of Micro-PET,to record the success rate of the image acquisition and the quality of the image.We then evaluated the strengths and weaknesses of the two groups of injections.Results The two groups showed statistical differences in puncture success rate,puncture time,drug residue,image quality and radioactive residues in the tail of mice (P<0.05 ).In a comprehensive comparison,the homemade indwelling needle was easy to construct and operate,with a high puncture success rate and little damage to the caudal vein of the mouse,and was superior in terms of the success rate and quality of image acquisition.Conclusion The combination of homemade tail vein needle and optimized experimental procedure can significantly improve the success rate and image quality of mouse tail vein puncture,which can be well applied to 13N-NH3-H2O myocardial perfusion Micro-PET imaging in mice.
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