A Comparison of the Effect of Different Anticoagulants on Portable Blood Glucose Mete
Objective Using hexokinase method as a reference method,to evaluate the accuracy and precision of different anticoagulants in the comparison of German Roche excellent glucose meter,and to further identify the most suitable anticoagulant.Methods Five tubes of heparin sodium (green head tube),sodium fluoride (grey head tube),EDTA-K2 (purple head tube)and sodium citrate (blue head tube)were extracted and prepared.These samples were then divided into two parts on average:one part was tested twice by biochemical analyzer (Toshiba 120),and the average value of the two biochemical analyzer test results was taken as the target value;the second part was tested by blood glucose meter.The accuracy of 26 Roche portable blood glucose meters was evaluated by comparing the mean value with the target value.POCT blood glucose meter was used to measure the same sample for 20 times to evaluate its precision.Results The standard deviation (s)of heparin sodium anticoagulant samples ranged from 0.12 to 0.38mmol/L,while coefficient of variation (CV)ranged from 2.04％ to 3.87％,and the precision qualification ratio was 100％.Compared with the target value,the qualified rate was 80％,and the qualified ratio of accuracy was 100％.The standard deviation (s)and coefficient of variation (CV)of sodium fluoride anticoagulation samples were 0.13-0.96mmol/L,2.24％-7.80％,and the precision qualification ratio was 80％.Compared with the target value,the qualified rate was 100％,and the qualified ratio of accuracy was 100％.The standard deviation (s)and coefficient of variation (CV)of EDTA-K2 anticoagulation samples were 0.19 -0.46mmol/L,2.42％-3.78％,and the precision qualification ratio was 100％.Compared with the target value,the qualified rate was 100％,and the qualified ratio of accuracy was 100％.The standard deviation (s)of sodium citrate anticoagulant test samples ranged from 0.11 to 0.27mmol/L,while coefficient of variation (CV)ranged from 2.34％ to 4.03％,and the precision qualification ratio was 100％.Compared with the target value,the qualified rate was 80％,and the qualified ratio of accuracy was 100％.Conclusion The accuracy and precision of the four anticoagulants used in portable blood glucose meter meet the requirements and can all be used for comparison.
万方数据
维普
