The Detection of Pathogenic Microorganismsand Drug Susceptibility Test in Stool Samples of 127 Cases of Bacterial Diarrhea
Objective To investigate the detection performance of pathogenic microorganisms and the results of drug sensitivity test in stool samples of suspected bacterial diarrhea.Methods A 1-year investigation was carried out in our hospital for this study.127 patients with suspected bacterial diarrhea admitted to our hospital from January,2023 to October,2023 were selected.8mL stool samples were collected for pathogen detection,and the detection and distribution of strains were recorded.The drug susceptibility test was performed to evaluate the drug resistance of pathogenic bacteria by the diffusion method with microbial susceptibility test paper.Results(1)Of 127 suspected bacterial diarrhea patients,125 cases were preliminarily screened for bacteria,of which 82 were negative and 43 were positive,including 7 cases of Vibrio para-hemolyticus,11 cases of salmonella,3 cases of salmonella and other bacteria,2 cases of pathogenic Escherichia coli,1 case of pathogenic Escherichia coli and other bacteria,19 cases of other bacteria.There were certain differences between the confirmed results of the district and the city,and the confirmed results of the city were correct after final review.With these steps,a total of 42 strains were detected.(2)Of 127 suspected bacterial diarrhea patients,24 were excluded,64 were negative,39 were positive,and a total of 41 strains of virus were detected.(3)The results of seasonal distribution showed that the bacterial detection rate was the highest from June to August,followed by March to May.The virus detection rate was the highest from September to October,followed by January to February,and there were significant differences in the detection rates of bacteria and viruses in different seasons(P＜0.05).(4)There were no significant differences in the detection rates of bacteria and viruses between different genders(P＞0.05).(5)Age distribution results showed that the detection rates of bacteria and viruses were higher in children and elderly groups,and the detection rates of bacteria and viruses were significantly different in different age groups(P＜0.05).(6)Drug sensitivity test showed that the resistance rate of salmonella to ciprofloxacin was the highest(50.00％),followed by ceftazidime(42.86％).The resistance rate of Vibrio parahemolyticus to cefazolin was the highest(71.43％),followed by ceftazidme,ceftriaxone,gentamicin and cefuroxime(57.14％).The resistance rate of pathogenic Escherichia coli to ciprofloxacin was the highest,reaching 100.00％.The resistance rate of other bacteria to cefuroxime and cefazolin was the highest(30.00％),followed by gentamicin(25.00％).Conclusion In 127 cases of suspected bacterial diarrhea,salmonella bacteria and norovirus are more common,and bacterial infection occurs frequently in summer and viral infection occurs frequently in autumn and winter.Children and the elderly are the main incidence groups,suggesting that clinical attention should be paid to these patients.For bacterial diarrhea,sensitive drugs can be combined with drug sensitivity test to enhance the efficacy.
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