Clinical Applications and Challenges of Digital PCR Technology in Methylation Detection of Tumor Liquid Biopsy
Digital PCR technology has recently demonstrated its unique advantages in the detection of methylation markers in liquid biopsy,with a high sensitivity and precise quantitative capabilities,making it a powerful tool for the early tumor diagnosis and monitoring of treatment effects.Through its ability of high-throughput and high-precision analysis of methylation status in various biological samples,digital PCR can improve the probability of the early tumor detection and provide an important reference basis for the development of personalized treatment strategies.Despite these advantages,applications of digital PCR technology in methylation detection still face some challenges,including how to ensure the accuracy and reproducibility of the detection,further improve the efficiency of sample processing procedures,and optimize the data analysis and result evaluation system.In addition,the establishment of industry standards that match national standards is also a key to facilitate the widespread clinical application of digital PCR technology.With continuous technological advancement and improvement,digital PCR is expected to become the"gold standard"for methylation detection in liquid biopsy,providing cancer patients with more accurate and rapid diagnostic tools,thus improving the efficiency of tumor diagnosis and treatment,as well as patient prognosis.
Digital PCRMethylationLiquid biopsyTumorEarly screening
NETL
NSTL
万方数据
