HPV阳性女性中宫颈液基细胞学、DNA倍体分析及P16/Ki-67双染检测对宫颈癌前病变的分流效果研究
A Study on the Triage Effect of Cervical Liquid-based Cytology,DNA Ploidy Analysis,and P16/Ki-67 Dual Staining in Cervical Precancerous Lesions in HPV-positive Women
关春艳 1董格红 1陈柯霖 2李佳 1冯力民 3廖柯鑫 3李少祥 1李洪利 1张国军2
作者信息
- 1. 首都医科大学附属北京天坛医院病理科,北京 100070
- 2. 首都医科大学附属北京天坛医院实验诊断中心,北京 100070
- 3. 首都医科大学附属北京天坛医院妇产科,北京 100070
- 折叠
摘要
目的 探讨在HPV阳性女性中,液基细胞学、DNA倍体分析及P16/Ki-67双染检测对宫颈癌前病变的分流作用.方法 回顾性分析2021年5月至2022年12月在我院妇科行阴道镜及宫颈活检的妇女590例.患者高危人乳头瘤病毒(human papilloma virus,HPV)检测阳性,且行液基细胞学(liquid-based cytology,LBC)、DNA 倍体分析、P16/Ki-67 双染 3 种检查,对上述3种方法的灵敏度、特异性、受试者工作特征(receiver operating characteristic,ROC)曲线进行统计分析.结果 液基细胞学、DNA倍体分析和P16/Ki-67双染3种筛查方法对宫颈癌前病变的灵敏度分别为84.2％、77.5％、76.4％,特异性分别为 40.7％、49.2％、70.1％,曲线下面积(area under the curve,AU C)分别是 0.625、0.634、0.733,其中,P16/Ki-67双染检测显著优于液基细胞学检查及DNA倍体分析(P＜0.0001).结论 本研究认为,在HPV阳性女性中,P16/Ki-67双染检测的分流效果最佳.
Abstract
Objective To investigate the triage effect of liquid-based cytology,DNA ploidy analysis,and P16/Ki-67 dual-stain immunohistochemistry in HPV-positive women with cervical precancerous lesions.Methods A retrospective analysis was conducted on 590 women who underwent vaginal colposcopy and cervical biopsy at our hospital from May,2021 to December,2022.All patients tested positive for high-risk human papillomavirus(HPV)and underwent three examinations:liquid-based cytology(LBC),DNA ploidy analysis,and P16/Ki-67 dual-stain.The sensitivity,specificity,and receiver operating characteristic(ROC)curves of the three methods were statistically analyzed.Results The sensitivities of liquid-based cytology,DNA ploidy analysis,and P16/Ki-67 dual-stain for cervical precancerous lesions were 84.2％,77.5％,and 76.4％,respectively,while the specificities were 40.7％,49.2％,and 70.1％,respectively.AUCs were 0.625,0.634,and 0.733,respectively.Among them,P16/Ki-67 dual-stain was significantly superior to LBC and DNA ploidy analysis(P＜0.0001).Conclusion This study suggests that P16/Ki-67 dual-stain has the best triage effect in HPV-positive women.
关键词
宫颈癌前病变/初筛/DNA倍体分析/P16/Ki-67双染/人乳头瘤病毒/液基细胞学Key words
Cervical precancerous lesions/Primary screening/DNA ploidy analysis/P16/Ki-67 dual-staining/Human papillomavirus/Liquid-based cytology引用本文复制引用
基金项目
北京市自然科学基金(J200002)
北京医管中心人才培养计划"登峰"项目(DFL20220505)
北京市高层次公共卫生技术人才培养计划(学科带头人-01-13)
出版年
2024
NETL
NSTL
万方数据