外周全血细胞IFN-γ和IL-2双因子检测在儿童结核诊断中的研究
The Study of IFN-γ and IL-2 Dual Factor Detection in the Diagnosis of Pediatric Tuberculosis
丁磊 1高媛媛 2周旭强 1何萍 2徐俊驰3
作者信息
- 1. 苏州市吴江区儿童医院/苏州大学附属儿童医院吴江院区检验科,江苏苏州 215200
- 2. 苏州大学附属儿童医院园区总院检验科,江苏苏州 215000
- 3. 苏州市第五人民医院/苏州市结核病防治重点实验室,江苏苏州 215007
- 折叠
摘要
目的 探索儿童外周全血细胞7干扰素(interferon-γ,IFN-γ)、白细胞介素2(interleukin-2,IL-2)双因子检测在儿童结核病中的辅助诊断价值.方法 选取2022年6月至2023年12月苏州大学附属儿童医院高度疑似结核感染患儿89例,利用 10kD 培养滤液蛋白(culture filtrate protein 10,CFP10)和 6kD 早期分泌性抗原靶蛋白(early secretary antigenic target-6 kD,ESAT6)刺激受试者全血18～24h,收集培养物上清,采用ELISA法(迪澳双因子试剂盒)检测上清中IFN-γ、IL-2水平,并对检测结果进行分析.结果 结核感染患儿比例随着年龄增高而增加,在活动性结核组中,以大于6岁和小于2岁患儿为主,潜伏性结核感组中,以大于6岁年龄段患儿为主;迪澳双因子试剂盒诊断结核感染与安图试剂盒诊断结果Kappa值为0.785,表明两种方法的一致性较好;活动性结核与潜伏性结核感染患儿组IFN-γ、IL-2检测结果相比,差异均有统计学意义(P＜0.001);根据迪澳双因子结果的ROC曲线表明,IFN-γ和IL-2双因子联合诊断的灵敏度为91.0％,特异性为81.8％,AUC为0.880.结论 外周全血细胞IFN-γ和IL-2双因子联合检测在儿童结核病中具有较高的辅助诊断价值,对于ATB和LTBI的鉴别诊断也有一定的临床意义.
Abstract
Objective To explore the diagnostic value of IFN-γ and IL-2 in children with tuberculosis.Methods Between June,2022 and December,2023,89 children with high suspected tuberculosis infection were selected from the Children's Hospital affiliated to Soochow University.The whole blood samples were stimulated with culture filtrate protein 10(CFP10)and early secretory antigenic target-6 kD(ESAT6)for 18-24h,and the supernatant of culture was collected to detect levels of IFN-γ and IL-2 in serum by ELISA method(Define.TB).Results The proportion of children infected with tuberculosis increased with age.In the active tuberculosis group,patients were generally older than 6 years old or younger than 2 years old.In the latent tuberculosis group,patients were generally older than 6 years old;Kappa value of the diagnosis of TB infection by Define.TB and TB-IGRA was 0.785,indicating a good consistency between the two methods;There were significant differences in IFN-γ and IL-2 detection results between active tuberculosis and latent tuberculosis infection groups(P＜0.001);ROC curve analysis of the Define.TB showed that the sensitivity,specificity and AUC of the combined diagnosis of IFN-γ and IL-2 were 91.0％,81.8％and 0.880,respectively.Conclusion Combined peripheral whole blood cell IFN-γ and IL-2 dual-factor assay has a high diagnostic value in pediatric tuberculosis,also has good clinical significance for the differential diagnosis of ATB and LTBI.
关键词
结核分枝杆菌/酶联免疫法/活动性结核/潜伏性结核Key words
Mycobacterium tuberculosis/Elisa/Active tuberculosis/Latent tuberculosis引用本文复制引用
基金项目
苏州市卫生计生委科技项目(LCZX202116)
苏州市吴江区科教兴卫项目(WWK202215)
苏州市吴江区儿童医院青年研究项目(2021QN05)
出版年
2024
NETL
NSTL
万方数据