An Analysis of Treatment Strategies and Results for 44 Cases of Difficult Blood Types
Objective To analyze the processing strategy of difficult blood type and to further explore the significance of blood type gene detection in difficult blood type.Methods 44 difficult blood type samples were selected from Blood Transfusion Department of our hospital,and all blood samples were determined by serological identification and gene detection.Results The detection results of the serological experiments in 44 samples showed as follow:5 cases of ABO discrepancy in forward/reverse typing caused by allogeneic stem transplantation,9 cases of weakened antigen due to the disease or physiological factors,antibodies were diminished in 14 cases which led to ABO discrepancy in forward/reverse typing,self-agglutination in 6 cases,which resulted in ABO discrepancy in forward/reverse typing,6 cases of ABO discrepancy in forward/reverse typing were observed in protein interference,3 cases of ABO positive and negative inconsistency caused by homologous antibodies,and one case of RhD mutation.Among 44 difficult blood groups,8 cases of difficult blood types were not discriminated by serology,and two of them failed to perform blood group gene testing.The results of the blood group genes in the remaining six cases indicated that three cases had no subform mutated genes,and they were ABO*O.01.01/ABO*B.01,ABO*A1.02/B.01,ABO*O.01.02/B.01.Two cases of the ABO subtype were ABO*B.01(with28G)/ABO*O.01.02,ABO*BA.02/ABO*O.01.02.One case of Del type was RHD*01 EL.32/RHD*17.05.Conclusion For results that cannot be well explained by serological experiments,we can combine them with the blood type gene test to obtain correct blood types.This approach can provide more reliable ABO/RhD blood group reports for clinical practice and ensure the safety of clinical transfusion.
Difficult blood typeBlood type geneHypotypeSerological experiments
NETL
NSTL
万方数据
