The Application Value of Serum Interleukin-10 Level Combined with CARS and GDS Scale Scores in the Diagnosis of Autism Spectrum Disorder in Children
Objective To investigate the application value of serum interleukin-10 level,Childhood Autism Rating Scale and Gesell Developmental Diagnostic Scale score in the diagnosis of childhood autism spectrum disorder.Methods The clinical data and peripheral blood samples of 80 children aged 1-6 years old with suspected autism were collected for the study.An autism group and non-ASD group were identified by following the Diagnostic and Statistical Manual of Mental Disorders(5th Edition).Childhood Autism Rating Scale was used to assess the severity of these children's condition.Developmental levels of these children were evaluated by the Gesell Developmental Diagnostic Scale.Flow cytometry was used to detect the serum interleukin-10 level of the two groups,and the differences in the serum interleukin-10 level,the score of the Child Autism Rating Scale,and the five subdomains and average scores of the Gesell Developmental Diagnostic Scale were compared and evaluated between the autism group and the non-ASD group,as well as among children with autism of different genders and ages.Logistic regression was applied to analyze the correlation between serum interleukin-10,the average score of the Childhood Autism Rating Scale and the Gesell Developmental Diagnostic Scale and the incidence of ASD.The application values of serum interleukin-10,the score of the Childhood Autism Rating Scale and the average score of the Gesell Developmental Diagnostic Scale in the diagnosis of ASD were evaluated by the receiver operating characteristic curve.Results Scores of the Autism Rating Scale in the autism group were significantly higher than those in the non-ASD group,while the serum interleukin-10 level,the five subdomain scores and the average score of the Gesell Developmental Diagnostic Scale were significantly lower than those in the non-ASD group(P＜0.05).There was no significant difference in serum interleukin-10 levels in children with autism of different genders and ages(P＞0.05).The score of the Autism Rating Scale for female children with autism was significantly higher than that of male children(P＜0.05),while there was no significant difference in the score of Autism Rating Scale among children with autism of different ages(P＞0.05).The scores and average scores of the Gesell Developmental Diagnostic Scale in female children with autism were significantly lower than those in male children with autism(P＜0.05),and the scores of gross motor and fine motor plates decreased compared with those in male children,but the difference was not statistically significant(P＞0.05).The scores and average scores of the Gesell Developmental Diagnostic Scale in children aged 1-3 years were significantly higher than those in children with autism aged 3-6 years,while there were no significant differences in adaptability,fine motor,language,and personal-social plate scores compared with those of children with autism aged 3-6 years(P＞0.05).Serum IL-10 level,CARS scale score and average GDS scale score of children with ASD aged 1-6 years were identified as independent risk factors for the onset of ASD.AUCs of serum IL-10 level,CARS scale score and average GDS scale score in the diagnosis of ASD in children aged 1-6 years were 0.64,0.91 and 0.85,respectively,while AUC of the combination of these three indexes was 0.96,suggesting certain diagnostic values.Conclusion The condition of 1-6-year-old female children with autism is relatively more severe,and the development level of female children with autism is more obvious than that of male children.The decrease of serum interleukin-10 level in children with autism,the abnormal score of the Childhood Autism Rating Scale and the average score of the Gesell Developmental Diagnostic Scale can be related to the onset of autism.
Autism Spectrum DisorderInterleukin-10ScaleDiagnosisApplication value
万方数据
维普
