A Comparative Analysis of Three Different Methods for Detecting Anti-nuclear Antibodies
Objective The current study aims to evaluate and compare the performances of indirect immunofluorescence assay(IIF),line immunoassay(LIA),and magnetic bar code immunofluorescence assay(MBC)to detect anti-nuclear antibodies(ANAs),including performance difference and consistency.Methods A total of 97 samples from autoimmune disease(AID)patients and 43 samples from healthy individuals in Shijingshan Hospital were collected for the study.IIf,LIA and MBC methods were used to detect ANA in serum samples of these two groups(fifteen anti-nuclear antibodies were detected by LIA and MBC methods).x2 text and Kappa tests were used to compare the positive detection rates of these two groups by three assays and the positive detection rates of these three methods in AID group.We then evaluated the difference and the consistency of the fifteen ANAs items'positive results by comparing between LIA and MBC methods.Results The positive detection rates of AID group patients of the three assays were significantly higher than the those of the control group.In the AID group,the differences were not statistically significant when comparing the positive rates of ANA by the three methods.In the analysis of the 15 antibodies detected in the AID group by LIA and MBC,differences were statistically significant in 2 items,with one poor consistency,six fair consistencies and seven good consistencies.Conclusion The positive detection rates of ANA are high among the three assays.The total consistency of ANA-15 results between LIA and MBC methods is acceptable except the poor consistency in anti-CENP-B antibody item detection.The positive detection rate of anti-CENP-B antibody by LIA is higher than the one by MBC combined with the fluorescent centromere pattern.Proper assays should be arranged according to specific situations of the laboratory in order to avoid missed diagnosis and over-treatment,and IIF can still play an important role in labs as a classical gold standard method.
Autoimmune diseaseAntinuclear antibodyIndirect immunofluorescence assayLine immunoassayMagnetic bar code immunofluorescence assay
万方数据
维普
