首页|基于SSR小豆新品种(系)DNA指纹图谱构建及亲缘分类初探

基于SSR小豆新品种(系)DNA指纹图谱构建及亲缘分类初探

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为寻求鉴定小豆品种基因型的新途径,对不同地区来源的37个新品种(系)和12个品种资源进行了SSR分析.利用7对SSR引物扩增出的22个多态位点,对供试品种(系)进行了区分,初步构建出供试品种(系)DNA指纹图谱.聚类分析表明,当截值取0.37时,49个小豆材料可划分为7个类群.37个育成品种(系)全部聚在同一类群内,遗传差异相对较小;在育成品种类群内,可划分为8个具有不同特征的亚类群,聚类结果与品种(系)的系谱来源相吻合.地方品种资源内的遗传多样性较高,与育成品种间遗传距离较远.
DNA Fingerprint and Relative Relationship of New Varieties and Lines of Azuki Bean by SSR Markers
A selected set of 37 breeded varieties or lines of azuki bean (Vigna angularis) from Beijing University of Agriculture, Japan and Hebei and 12 germplasms from Beijing, Hebei, Tianjing, Sichuan, Jilin, Liaoning, Jiangsu in China and Janpan were tested by SSR markers. 22 polymorphic PCR product fragments were gained using 7 pairs of primers of SSR. DNA fingerprint of these varieties or lines were pre-marily constructed. The results of Cluster phylogenetic analysis indicated that all 49 accessions were classified into 7 groups, when the default 0. 37 parameters were used. 37 breeded varieties or lines were clustered in the same one group. 8 subgroups were divided into in the group of breeded varieties or lines, this result was consistent with the analysis of their family tree. There was more exiguous genetic diversity between breeded varieties or lines than landraces. The genetic distance of landraces was farther with the breeded.

Adzuki bean (Vigna angularis)breeded varietiesSSRfingerprint

赵波、金文林、濮绍京、郭蓓、万平

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北京农学院,植物科学技术系,北京,102206

小豆 育成品种 SSR指纹图谱

北京市教委人才强教项目北京市教委人才强教项目

PXM2007-014207-04453PXM2006-014207-021717

2008

北京农学院学报
北京农学院

北京农学院学报

CSTPCD
影响因子:0.539
ISSN:1002-3186
年,卷(期):2008.23(3)
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