Functional validation of MdSAUR71 and MdSAUR72 in regulating anthocyanin accumulation in apple fruit
[Objective]In order to understand the regulation mechanism of MdSAUR71 and MdSAUR72 on apple anthocyanin biosynthesis,[Methods]the apple calli of Malus domestica'Orin'was selected as the experimental materials.MdSAUR71 and MdSAUR72 were constructed into overexpression vectors and transformed into apple calli successfully.[Results]After 15 days of light and low temperature cultivated,the transgenic apple calli were displayed red color,which suggested that anthocyanin were accumulated in transgenic apple calli.Compared with wild type control,the expression of MdSAUR71 and MdSAUR72 genes and anthocyanin biosynthesis genes were significantly increased in transgenic calli by real-time quantitative PCR.[Conclu-sion]The results showed that MdSAUR71 and MdSAUR72 are participate in anthocyanin biosynthesis by coordinating the transcription of anthocyanin biosynthesis genes.
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