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条华蜗牛破厣前后体内总糖含量的变化

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[目的]了解条华蜗牛破厣前后体内总糖含量变化规律,为深入研究蜗牛破厣生化机理提供参考。[方法]用蒽酮-硫酸比色法测定了两个体质量组条华蜗牛在破厣、休眠、取食或饥饿等条件下足和内脏的总糖含量。[结果]条华蜗牛破厣后体内含糖量升高,体质量组Ⅳ蜗牛足、内脏含糖量都高于相应体质量组Ⅰ。破厣前蜗牛足内含糖量与破厣后的差异显著(P<0。05),而内脏的差异不显著。休眠蜗牛体内含糖量随休眠天数增加有降低的趋势,除体质量组Ⅰ蜗牛足内含糖量休眠1 d后(27。62 μg/mg)显著高于5 d~15 d外,其他休眠时间之间差异都不显著。蜗牛取食后,足和内脏含糖量变化趋势基本类似,都是取食12 h~24 h后增加,然后有所下降。除体质量组Ⅳ足外,取食24 h后都显著高于取食12 h和36 h。活动蜗牛体内含糖量随饥饿时间延长有降低趋势,饥饿36 h后含糖量均显著低于饥饿12 h。[结论]条华蜗牛破厣前后体内总糖含量变化与多个因素有关,总糖量变化规律反映了蜗牛破厣的生理生化特征。
Variation of total sugar content in the body of Cathaica fasciola before and after epiphragm-breaking
[Objective]To understand the variation characteristics of the total sugar content in the body of Cathaica fasciola be-fore and after epiphragm-breaking,and to provide information for further research on the biochemical mechanism of epiphragm-breaking.[Methods]The total sugar content in the feet and viscera in two weight groups of C.fasciola under dif-ferent conditions,including epiphragm-breaking,the duration of dormancy,feeding and starvation,were measured with the anthrone colorimetry.[Results]The total sugar content in the body of dormant snails increased after epiphragm-breaking,which was higher in the snail feet and viscera in weight group Ⅳ than that in weight group Ⅰ.There was a significant difference in the sugar content of snail feet between before and after epiphragm-breaking,while no significant difference was found in the viscera(P<0.05).The total sugar content in dormant snails decreased with the increase of dormant duration,the differences between dormant durations were not significant except for the significant differences between the dormant durations of 1 d(27.62 μg/mg)and 5 d-15 d in the feet of weight group Ⅰ.After feeding,the variation of the total sugar content in the feet and viscera had basically similar tendency,which all increased after feeding for 12-24 h then decreased after feeding for more than 24 h Except for the feel in weight group Ⅳ,the total sugar content after feeding for 24 h was significantly higher than those for 12 h and 36 h.The total sugar content in the feet and viscera of active snails decreased with the increase of starvation duration,which was significantly lower after being starved for 24 h than 12 h.[Conclusion]The variation of total sugar content in the body of C.fasciola before and after epiphragm-breaking was affected by many factors,which reflected the physiological char-acteristics in epiphragm-breaking.

Cathaica fasciolaepiphragm-breakingtotal sugar contentbody weightfeedingstarvation

张民照、覃晓春、赵晨、张爱环

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北京农学院生物与资源环境学院/农业农村部华北都市农业重点实验室,北京 102206

条华蜗牛 破厣 总糖含量 体质量 取食 饥饿

北京市农委项目

201603

2024

北京农学院学报
北京农学院

北京农学院学报

影响因子:0.539
ISSN:1002-3186
年,卷(期):2024.39(1)
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