Development of an indirect ELISA for detection of antibody to Torque teno sus virus type 1
[Objective]This study was aimed to establish a serological method for detection of Torque teno sus virus type 1(TTSuV1).[Methods]Using the capsid protein of TTSuV1 as coating antigen,the reaction conditions for antibody detection were screened and optimized to establish an indirect enzyme-linked immunosorbent assay(ELISA)for detection of the antibody against TTSuV1 capsid protein.Subsequently,its specificity and reproducibility test were conducted,and its preliminary clinical detection were performed.[Results]All the best reaction conditions of the indirect ELISA for the detection of the antibody to TTSuV1 capsid protein were determined.The detection results of 8 kinds of positive serum samples of swine-derived viruses showed that only positive serum samples of TTSuV1 was positive,showing good specificity.The coefficients of variation for in-tra-assay was under 5％,and that for inter-assay was under 10％,indicating good reproducibility.The detection results of 400 pig serum samples from Beijing and Hunan regions showed that the positive rate of antibodies against TTSuV1 capsid protein was 75.25％,and the positive rate of PCR was 71.00％,there is no significant(P＞0.05)difference between the two methods,and which indicated that there was a high rate of TTSuV1 infection in this two regions.[Conclusion]An indirect ELISA with good specificity and reproducibility for detecting antibodies to the TTSuV1 capsid protein was established,and it can be used for clinical detection of antibodies against TTSuV1.
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