Exploration of the effect of porcine secretory leukocyte protease inhibitor on skin injury healing in mice
[Objective]To investigate the therapeutic effect of porcine secreted leukocyte protease inhibitor(SLPI)on skin wound healing and explore its potential value.[Methods]This study used recombinant pig derived SLPI to treat mouse wounds,screened the optimal concentration of the protein,and then explored its anti injury healing effect at this concentration;The ex-periment was divided into trauma group,SLPI treatment group,and positive drug control group(Positive drug represents Bail-ingjinfangyijunye).The gross pathological changes of mouse wounds at different times and microscopic pathological changes were observed,and the clinical score of wound healing were used to evaluate the effect of SLPI on wound recovery.[Results]The results showed that the optimal concentration of SLPI was 0.25 mg/mL.The ulcerated area of the wound gradually de-creased over time in all three groups of mice.Compared with the trauma group,from day 3 onwards,the pathological scores of the SLPI treatment group were lower than those of the trauma group(P＜0.05 or P＜0.01),and the performance was similar to that of the positive drug group.At day 7,both the positive drug control group and SLPI group showed hair follicle growth,while changes in the injury group were not significant.Microscopic pathological changes showed that compared to the blank control group,the SLPI group and positive drug control group displayed epidermal layers and sebaceous glands on day 5,and on day 7,a significantly higher number of fibroblasts and reduced inflammatory cell infiltration were observed.[Conclusion]In summary,the recombinant expression of pig derived SLPI has a certain effect on mouse skin wound healing,providing experi-mental data for the development of effective drugs targeting pig skin injury.
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