[Objective]In order to study the effects of chromosome karyotype difference among different varieties of Marigold(Tagetes erecta L.).[Methods]Eight varieties of material treatments were as materials,which were'Rich yellow','Inca yellow','Discovery yellow','Discovery orange','Yellow moon of dream','Marvel golden',Marigold grandiflora,Chifeng Marigold'Yellow'.The karyotype formula,average arm length,relative length ratio of longest and shortest chromosomes,kar-yotype type,chromosome length composition,karyotype asymmetry coefficient and other karyotype parameters among different varieties were measured.[Results]The results showed that the karyotype asymmetry coefficients of the eight marigold varieties from large to small were Tagets erecta L.'Rich yellow'>Tagets erecta L.'Inca yellow'>Chifeng Marigold>Tagets erecta L.'Discovery yellow'>Tagets erecta L.'Yellow moon of dream'>Tagets erecta L.'Discovery orange'>Tagets erecta L.'Marvel golden'>Marigold grandiflora,Therefore,the evolutionary order of 8 varieties of marigold can be concluded as follows:Marigold grandiflora,Tagets erecta L.'Discovery orange',Tagets erecta L.'Yellow moon of dream',Tagets erecta L.'Discovery yellow',Chifeng Marigold,Tagets erecta L.'Inca yellow',Tagets erecta L.'Rich yellow'.[Conclusion]It also proved that marigold may be a relatively evolved species of marigold genus.