Pathogen(s)identification of tomato viral disease in Mafang Town,Pinggu District
[Objective]To clarify the pathogen of tomato viral disease in Mafang Town,Pinggu District,and provide a basis for the prevention and control of tomato virus disease in this area.[Methods]In October 2022,tomato leaves from Hebei Village,Guogezhuang Village,Zaolizhuang Village,Liyang Village,and Xiaotun Village showing symptoms of viral diseases including yellowing,fern leaves and chlorosis were collected and screened of potential viruses by small RNA deep sequencing,and the i-dentified viruses were further verified by PCR/RT-PCR.[Results]The main virus species infecting tomato plants were cucum-ber mosaic virus(CM V),southern tomato virus(STV),tomato chlorosis virus(ToCV)and tomato yellow leaf curl virus(TYLCV).The detection rates of the corresponding virus in the 12 samples were 58.3％,16.7％,16.7％,and 100％,and the mixed infection rate of two or more viruses was 58.3％,by performing full-genome amplification and constructing phylogenetic trees of 5 TYLCV,it was indicated that all of them are most closely related to the TYLCV isolates from Beijing.[Conclusion]The viral disease was widespread and severe in Mafang town,and the test results indicated that TYLCV was the main virus in-festing local tomatoes,and mixed infection is common.
facilitytomatovirus diseasepathogen identificationsmall RNA deep sequencing
万方数据
维普
