[Objective]In order to reveal the molecular mechanism of miR395a-MdWRKY26 expression pattern downregulating salt tolerance in apples under salt treatment,and to provide a basis for the breeding of new salt tolerant apple varieties.[Meth-ods]Wild type apple seedlings of'Gala'and transgenic apple seedlings of miR395a-OE,miR395a-RNAi,MdWRKY26-OE,and MdWRKY26-RNAi obtained through stable genetic transformation were used as experimental materials.They were treated with 200 mM NaCl to observe the phenotype and detect the expression level under salt treatment using RT qPCR.The content of malondialdehyde was measured using UV spectrophotometer.[Results]The results showed that after 200 mM NaCl treat-ment,compared with the control,miR395a-OE and MdWRKY26-RNAi transgenic plants were more susceptible to damage and were more sensitive to salt;The damage to the MdWRKY26-OE and miR 395a RNAi transgenic plants was relatively mild,indi-cating that the MdWRKY26-OE and miR395a RNAi transgenic plants have salt tolerance.[Conclusion]Through salt tolerance analysis,it was found that the miR395a gene negatively regulates salt tolerance in apples,while the MdWRKY26 gene plays a positive regulatory role.The molecular mechanism of miR395a-MdWRKY26 regulating salt tolerance in apples has been re-vealed,which can weaken the damage to plants under salt treatment.
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