Cardiac clinical symptoms and pathological changes in experimentally PRRSV-infected piglets
[Objective]To comprehend the heart damage of piglets caused by PRRSV infection.[Methods]Landrace piglets about 5 weeks old were artificially infected with PRRSV JXA1 strain.After the onset of obvious clinical symptoms,the heart sounds of piglets were diagnosed by a stethoscope.At three days post infection,piglets were autopsied and the ventricular parie-tal tissues were sampled to prepare the paraffin sections.The pathological changes of piglet hearts were detected by the Hema-tein-Eosin staining,and the PRRSV N protein were determined by immunohistochemical staining.[Results]Cardiac auscultation showed that the second heart sound of infected piglets was significantly enhanced,and there were tachycardia and arrhythmia and occasional pericardial patting sounds in some infected piglets.The cardiac autopsy showed that the slightly thickened pericardium,small amount of fluid in pericardial space,the slightly larger transverse cardiac diameter,the softer tis-sues,and some myocardial hemorrhagic spots in infected piglets.HE staining indicated myocardial tissue congestion,lymphocyte infiltration and granular degeneration,etc.Immunohistochemical staining indicated that PRRSV N protein was widely detected in myocardial tissue,not only in myocardial cells,but also in vascular endothelial cells.[Conclusion]Three days post infection with PRRSV JXA1 strain,cardiac function changes and pathological changes of myocardial tissues were directly caused,which suggests the cardiac function protection should be strengthened as soon as possible in the treatment of PRRSV-infected piglets.
porcine reproductive and respiratory syndrome viruscardiac auscultationvascular endothelial cellspathological changecongestion
万方数据
维普
