Extraction,purification and immunogenicity of β2-glycoprotein inbovine blood
[Objective]To add a new way to the acquisition of Bβ2GPI,improve the utilization value of bovine blood,and improve the waste of bovine blood resources to a certain extent.[Methods]Initial precipitation with perchloric acid was followed by fur-ther purification using Heparin Sepharose CL-6B affinity chromatography and zeolite.The purity of the extracted Bβ2GPI was identified and analyzed by gel electrophoresis,and then the immunogenicity of Bβ2GPI was evaluated by Enzyme-linked immu-nosorbent assay(ELISA).[Results]Experiments showed that the purity of the extracted and purified Bβ2GPI was about 95％,and its reactivity was the same as that of the β2GPI standard.[Conclusion]Bβ2GPI was successfully extracted and purified from bovine blood,and the obtained protein had high purity and good immunogenicity.
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