Studies on callus tissue induction and subculture from different explants of Quercus dentata
[Objective]In this study,the roots and leaves of Quercus dentata were used as explants to screen the optimal condi-tions of callus tissue induction and subculture,which can support the establishment of tissue culture and rapid propagation sys-tem for Q.dentata.[Methods]In the WPM,MS and 1/2MS medium containing different concentrations of NAA and 6-BA,the explants of roots and leaves from.Q.dentata seedling were cultured to induce callus tissue,and the induction rates were recor-ded,analyzed,and compared.Then the induced callus tissue from roots was inoculated into WPM medium with different concen-trations of 2,4-D and 6-BA to proliferate,and the proliferation coefficients were calculated and compared.[Results]The optimal medium for the induced callus tissue of leaves was MS medium containing 1.0 mg/L NAA and 0.5 mg/L6-BA.The optimal medium for the induced callus tissue of roots was WPM medium containing 2.0 mg/L NAA and 1.0 mg/L 6-BA.After com-parison,the induction rate of root explants was higher than that of leaves.WPM medium containing 1.2 mg/L 2,4-D and 0.9 mg/L 6-BA was found to be optimal for callus tissue proliferation.[Conclusion]In this study,the optimal medium formulation for the induction and proliferation of Q.dentata callus tissue was successfully screened out,which laid the foundation for estab-lishing tissue culture and rapid propagation system of Q.dentata.
万方数据
维普
