Cloning and expression analysis of V-ATPase-D subunit gene of Tetranychus cinnabarinus
[Objective]The study aimed to identify effective biological approaches for controlling Tetranychus cinnabarinus.[Methods]The V-ATPase-D gene was cloned and its gene sequence was analyzed;real-time quantitative fluorescence PCR was used to characterize the expression of V-ATPase-D.[Results]The full length of the cloned V-ATPase-D subunit gene(GenBank accession number:OR836605)is 956 bp,with an open reading frame of 744 bp encoding 247 amino acids.The expression of V-ATPase-D was highest in the adult mite stage followed by the egg stage,and lowest in the nymph and larva stages.[Conclu-sion]The V-ATPase-D gene was cloned and its expression characteristics at various stages were clarified,which may provide basis for the development of new biopesticidal acaricides targeting the V-ATPase in the future.
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