朱砂叶螨V-ATP酶D亚基基因克隆与表达分析
Cloning and expression analysis of V-ATPase-D subunit gene of Tetranychus cinnabarinus
耿文 1王谦稳 1胡祺凡 1陈佳雯 1卜春亚1
作者信息
- 1. 北京农学院生物与资源环境学院/农业农村部华北都市农业重点实验室,北京 102206
- 折叠
摘要
[目的]旨在寻找防治朱砂叶螨有效的生物学办法.[方法]克隆出朱砂叶螨V-ATP酶D亚基(V-AT-Pase-D)基因,并对该基因进行了生物学特征分析;采用荧光定量PCR技术,对朱砂叶螨其生命周期中V-AT-Pase-D基因表达量进行了测定.[结果]克隆出朱砂叶螨V-ATPase-D(GenBank登录号:OR836605).朱砂叶螨V-ATPase-D基因全长956 bp,开放阅读框744 bp,编码247个氨基酸,V-ATPase-D在朱砂叶螨的不同生长发育阶段均表现出了基因活性,其中在成螨期内展现出最高的表达水平,其次是卵阶段,而若螨与幼螨阶段则呈现出较低的基因表达水平.[结论]成功克隆出朱砂叶螨V-ATPase-D基因,并明确其在朱砂叶螨生长过程中不同时期内的基因表达活性,为进一步探索基因的作用机制同时也对以V-ATP酶为靶标的新型生物杀螨剂提供了研究基础.
Abstract
[Objective]The study aimed to identify effective biological approaches for controlling Tetranychus cinnabarinus.[Methods]The V-ATPase-D gene was cloned and its gene sequence was analyzed;real-time quantitative fluorescence PCR was used to characterize the expression of V-ATPase-D.[Results]The full length of the cloned V-ATPase-D subunit gene(GenBank accession number:OR836605)is 956 bp,with an open reading frame of 744 bp encoding 247 amino acids.The expression of V-ATPase-D was highest in the adult mite stage followed by the egg stage,and lowest in the nymph and larva stages.[Conclu-sion]The V-ATPase-D gene was cloned and its expression characteristics at various stages were clarified,which may provide basis for the development of new biopesticidal acaricides targeting the V-ATPase in the future.
关键词
朱砂叶螨/V-ATP酶/克隆/荧光定量/PCRKey words
Tetranychus cinnabarinus/V-ATPase/gene cloning/fluorescence quantitative PCR引用本文复制引用
基金项目
国家自然科学基金资助项目(31670648)
北京市自然科学基金资助项目(6212004)
出版年
2024
NETL
NSTL
万方数据