Cloning and functional exploration of the heterologous expression of MdNAC56-L in Malus plants
[Objective]To investigate the function of the MdNAC56-L gene in the chlorophyll biosynthesis pathway in apple.[Methods]We used'Orin'apple(Malus domestica'Orin')as the experimental material.MdNAC56-L gene was cloned by RNA extraction and reverse-transcription.Constructing overexpression vectors and silencing vectors for MdNAC56-L,and in-stantaneously overexpressing and silencing the MdNAC56-L gene in wild-type tobacco.Phenotypic analysis was performed on transgenic leaves,chlorophyll content was measured using spectrophotometry,and the expression of the key gene NtPAO in the chlorophyll degradation pathway was identified using fluorescence quantitative PCR.The biological function of the MdNAC56-L gene was preliminarily analyzed.[Results]The results showed that compared with wild-type tobacco,tobacco leaves overex-pressing the MdNAC56-L gene showed a significant decrease in chlorophyll content and a significant increase in the expression of the key gene NtPAO for chlorophyll degradation pathway.Tobacco leaves silencing the Md NAC56-L gene showed no signif-icant increase in chlorophyll content and no significant decrease in the expression of the key gene NtPAO for chlorophyll degra-dation pathway.[Conclusion]MdNAC56-L may be involved in the positive regulation of chlorophyll degradation in the chloro-phyll synthesis pathway.
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