Establishment of regeneration and genetic transformation system of'Benihoppe'strawberry shortened stem
[Objective]In order to obtain regenerated and genetically transformed plants of the'Benihoppe'strawberry.[Meth-ods]The shortened stems of octoploid strawberry(Fragaria ×ananassa Duch.'Benihoppe')were used as experimental mate-rials.The targeted sequences of FaTFL1 and FaSOC1 known to suppress flowering process of'Benihoppe'strawberries under long-day conditions,were incorporated into the CRISPR/Cas9 vector pDIRECT-22C.FaTFL1 and FaSOC1 gene edited plants were obtained by establishing shortened stem regeneration and genetic transformation system of'Benihoppe'strawberry.[Re-sults]The shortened stem is directly induced to form adventitious buds without callus generated,and the whole regeneration cy-cle is about two months.By optimizing the medium conditions,a genetic transformation system mediated by Agrobacterium tu-mefaciens was established.The Cas9 elements of the CRISPR/Cas9 vector of genetically transformed strawberry plant was i-dentified,resulting in a positivity rate of 18.62％.[Conclusion]A highly efficient regeneration system utilizing the shortened stem bases of octoploid'Benihoppe'strawberry tissue culture seedlings as experimental materials,along with an Agrobacteri-um-mediated genetic transformation system,has been established.
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