Distribution characteristics of fungi in soil aggregates of greenhouse soil
[Objective]The aim of this study is to explore the distribution characteristics of fungi in different soil aggregates of greenhouse soil and ultimately clarify the ecological adaptation mechanism of fungi in soil micro-environment.[Methods]The changes in soil aggregate properties in greenhouse and open soil were compared,and the abundance and community structure of fungi in different soil aggregates were evaluated by quantitative PCR and high-throughput sequencing.[Results]The pH of greenhouse soil decreased by 0.68 units compared with open soil,while the conductivity,soil organic matter and dissolved or-ganic carbon contents were increased by 30.6％,85.8％and 94.1％,respectively.The DOC content in aggregates with particle size below 0.5 mm was significantly higher than that in aggregates with particle size greater than 0.5 mm.The EC value of mi-cro-aggregates(＜0.25 mm)was significantly lower than that of macro-aggregates(2-0.25 mm)and mega-aggregates(＞2 mm).Through amplification and sequencing of ITS gene,OTU numbers obtained from greenhouse soil were much more than that in open soil.There were highest and lowest specific OTU numbers in the aggregates with particle size below 0.25 mm and 0.5-0.25 mm size,respectively.The greenhouse environment significantly reduced the abundance of ITS gene,while increased the α-diversity of fungal community compared with open soil.Meanwhile,the α-diversity of aggregates with 0.5-0.25 mm par-ticle size was the lowest among all aggregates(P＜0.05).Moreover,principal coordinate analysis showed that the community structure of fungi in greenhouse environment and aggregates with 0.5-0.25 mm particle size were differed from that in open soil and other aggregates.The predominant fungi in soil were Mortierellomycota and Ascomycota at phylum level,while the relative abundances of Basidiomycota and Glomeromycota in soil were significantly higher than those in the open soil.[Conclusion]Compared with the open soil,the abundance of fungi was evidently decreased under greenhouse condition,while the diversity was increased.Redundancy analysis showed that pH was the key factor affecting the change of fungal community structure in greenhouse soil.The dominant fungi in the greenhouse soil were Mortierellomycota,Ascomycota,Basidiomycota and Glomero-mycota at phylum level.Moreover,the community structures of fungi were significantly distinct among different levels of aggre-gates,especially in aggregates with 0.5-0.25 mm particle size.Redundancy analysis revealed that DOC was the key factor driv-ing the difference among different levels of aggregates.
greenhouse soilaggregatefungal community structurehigh-throughput sequencing
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