Effect of Pb on pollen germination and tube growth in'Zaosu'pear
[Objective]To provide a theoretical basis for revealing the characteristics and mechanism of heavy metal pollution af-fecting the growth and development of Pyrus spp.pollen tubes.[Methods]Pollen was cultured in medium including 0 mg/L,0.1 mg/L,1 mg/L,10 mg/L,50 mg/L,and 100 mg/L Pb respectively,then the germination rates of pollen and the length of pollen tubes were analyzed.The components of pollen tube wall were also observed using fluorescent labeling.[Results]The re-sults showed that Pb inhibited pollen germination and tube growth in a concentration dependent manner.Pb also affected the distribution of the cell wall components.In the control group,cellulose was uniformly present throughout pollen tube wall,cal-lose was gradually reduced toward the tip,acidic pectin was uniformly distributed on pollen tube wall,and esterified pectin was enriched at the tip and the subapical part of the pollen tubes.0.1 mg/L Pb did not significantly affect the distribution of pollen tube cell wall components.After treatment with 100 mg/L Pb,cellulose and callose were accumulated respectively at the base and tip of pollen tube wall,acidic pectin decreased,and esterified pectin was distributed only at the tip of pollen tube wall.[Con-clusion]100 mg/L Pb significantly inhibited pollen germination and pollen tube growth,as well as influenced the distribution of pollen tube cell wall components.But 0.1 mg/L Pb did not significantly affect Pyrus spp pollen germination and tube growth.
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