Early evaluation of fruiting traits and the field verification of morel mushroom strains
[Objective]This study establishes an early evaluation system for the fruiting traits of morel mushroom strains.This is achieved through the examination of growth characteristics in stock culture and mother spawn strains,fruiting tests in an arti-ficial climate chamber,and verification experiments conducted in field cultivation.The findings will serve as a foundation for fu-ture cultivation practices.[Methods]Ten commercial strains of Morchella sextelata were used.The growth characteristics,in-cluding mycelial growth rate and biomass,were measured in both stock culture and mother spawn media.The conditions of my-celial growth and sclerotia during the fruiting process were observed using artificial climate chambers under three temperature modes.Furthermore,the fresh weight of individual mushrooms,pileus length,pileus diameter,yield,and other agronomic traits were documented from field cultivation experiments.[Results]Significant differences were observed among the strains in the stock culture and mother spawn media,and fruiting tests.By assigning scores to each of the three experiments and summing them up,the strains AK,G8 and JYN emerged as the highest-scoring candidates,suggesting their potential for superior yield.Field validation results showed that the strains AK,G8 and JYN performed outstandingly in terms of yield,fresh weight of indi-vidual mushrooms,and pileus length,which are consistent with the predicted results.[Conclusion]An early evaluation system of the quality of morel mushroom strains can be conducted based on the comprehensive performance of growth characteristics of the stock culture and mother spawn strains,and fruiting tests in an artificial climate chamber.This provides technical support for strain selection and quality evaluation in practical production.