Development and application of ancient tree genome database
[Objective]To establish an ancient tree genome database for storing genetic information data of ancient tree and relat-ed species,and to achieve browsing,querying,and online analysis of genome data.[Methods]According to the relevant provi-sions of the"Technical Specifications for the Survey of Ancient Trees and Famous Trees",trees aged 100 years or older are con-sidered ancient trees.In this study,registered ancient tree species were referenced to collect genetic information of the same spe-cies of ancient trees published worldwide from relevant databases.The collected ancient tree genomes were organized,corrected,and annotated for gene function,and genetic data were organized and analyzed.The standardized data were uploaded to the de-veloped ancient tree genome database.[Results]The ancient tree genome database was developed and launched online,enabling storage,querying,and browsing of genetic data such as ancient tree genomes.Currently,genetic and comparative genomic data of 89 species of ancient trees and related species have been collected,providing data support for studying the molecular mecha-nisms of longevity under complex environmental stresses in ancient trees.Online data mining tools have been developed,reali-zing functions such as sequence alignment based on BLAST,batch sequence extraction,enrichment analysis of GO and KEGG,and collinearity analysis,providing a data analysis platform for studying excellent genes in ancient trees.[Conclusion]The com-pilation and summarization of genetic information of ancient tree and related species genomes,and the development and applica-tion of the ancient tree genome database,have greatly facilitated scientific researchers in ancient tree research,providing new in-sights for effectively conserving ancient tree resources.
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