Objective To explore the application performance of sequencing reagent QzTGS HLA MX9 (TGS sequencing) based on Nanopore platform in human leukocyte antigen (HLA) genotyping.Methods A total of 48 samples of routine HLA typing in Beijing Red Cross Blood Center Laboratory were selected.All samples were genotyped by PCR-SBT and TGS respectively.The genotyping results and performance of the two methods were compared.Results The results of gene detection of HLA-A,-B,-C,-DRB1 and-DQB1 in 48 samples by TGS sequencing method and PCR-SBT sequencing method were completely consistent at high resolution level.TGS sequencing method could directly specify new genes and single combination typing results for all samples.The results of HLA-A,-B,-C,-DRB1 and-DQB1 by TGS and PCR-SBT in 48 samples were completely consistent,and the new genes could be directly designated by TGS and the samples designated as a single combination were 48 cases (100%).TGS had obvious advantages in terms of time-consuming,new gene determination,reading length,machine maintenance,ambiguity,and real-time data.Conclusions Compared with PCR-SBT,QzTGS HLA MX9 based on Nanopore sequencing platform has higher accuracy and less time-consuming.
关键词
人类白细胞抗原/Nanopore测序/PCR-SBT/基因分型
Key words
human leukocyte antigen (HLA)/Nanopore sequencing/PCR-SBT/genotyping
维普
万方数据