Evaluation of sequencing reagent QzTGS HLA MX9 based on Nanopore platform in HLA genotyping
Objective To explore the application performance of sequencing reagent QzTGS HLA MX9 (TGS sequencing) based on Nanopore platform in human leukocyte antigen (HLA) genotyping.Methods A total of 48 samples of routine HLA typing in Beijing Red Cross Blood Center Laboratory were selected.All samples were genotyped by PCR-SBT and TGS respectively.The genotyping results and performance of the two methods were compared.Results The results of gene detection of HLA-A,-B,-C,-DRB1 and-DQB1 in 48 samples by TGS sequencing method and PCR-SBT sequencing method were completely consistent at high resolution level.TGS sequencing method could directly specify new genes and single combination typing results for all samples.The results of HLA-A,-B,-C,-DRB1 and-DQB1 by TGS and PCR-SBT in 48 samples were completely consistent,and the new genes could be directly designated by TGS and the samples designated as a single combination were 48 cases (100%).TGS had obvious advantages in terms of time-consuming,new gene determination,reading length,machine maintenance,ambiguity,and real-time data.Conclusions Compared with PCR-SBT,QzTGS HLA MX9 based on Nanopore sequencing platform has higher accuracy and less time-consuming.
human leukocyte antigen (HLA)Nanopore sequencingPCR-SBTgenotyping
万方数据
维普
