Application and evaluation of mice model with Qi deficiency and blood stasis syndrome
Objective To explore the expansion of the inspection index by taking the syndrome of qi deficiency and blood stasis as the representative, and provide the basis for the feasibility of syndrome diagnosis of model animals. Methods A total of 30 mice were randomly assigned to the blank control group,qi deficiency and blood stasis group,and the herbal counterfactual group, with 10 mice in each group. The mice in both qi deficiency and blood stasis group and the herbal counterfactual group were subjected to a modeling protocol involving starvation, fatigue, exposure to cold and dampness for a duration of 20 days. In the herbal counterfactual group, the mice received a daily oral gavage of concentrated Astragalus-Curcuma decoction, starting from the third day of modeling. Conversely, the mice in the blank control group and the qi deficiency and blood stasis group were gavaged with the same volume of distilled water daily for 18 days. The general condition and body mass of the mice was observed continuously. At the end of the modeling period, the water content of feces and appetite for food were assessed. Additionally, the behavioral characteristics of the mice in each group were evaluated using a wind avoidance test, mechanical pain field assessment, and open field test. Results Compared with the blank control group, the body weight, the staying time in the 2nd and 3rd zones in the shelter experiment, the total distance, the average speed, the times of crossing the grid in the open field experiment, the mechanical pain zone and the R, G and B values of the tongue color of the mice in the Qi deficiency and blood stasis groups and the Chinese medicine counter-evidence group were all lower (P<0.05,P<0.01). Compared with the group of qi deficiency and blood stasis, the body weight, the staying time in the second and third regions in the shelter experiment, the total distance, the average speed, the times of crossing the grid in the open field experiment and the R,G,B values of tongue color were all higher in the group of TCM (P<0.05,P<0.01). Conclusion The indexes of shelter from the wind and mechanical pain field mice, as well as the indexes of open shelter experiment, fecal moisture content and tongue color detection, can be used as extended inspection indexes for diagnosing qi deficiency and blood stasis syndrome in mice, but foraging desire cannot be used as an effective index.
Qi deficiency and blood stasis syndromeanimal modelexpanded inspection diagnosissyndromes study
万方数据
维普
