Effect and mechanism of Bufei Huoxue Capsule on the intervention of chronic inflammation and mucus hypersecretion in the airways of rats with chronic obstructive pulmonary disease
Objective To investigate the intervention effect and mechanism of Bufei Huoxue Capsule on airway inflammation and mucus hypersecretion in rats with chronic obstructive pulmonary disease(COPD).Methods The rat model of COPD was established by smoking and intratracheal instillation of lipopolysaccharide.The lung function was measured by small animal lung function meter,and the morphology of airway epithelial cells was detected by hematoxylin-eosinstaining and alicinblue-periodopho staining,the expressions of IL-1β,IL-6,NE,TGF-β,EGF,IL-13 and TNF-α in BALF were detected by enzyme-linked immunosorbent assay,the protein expressions of TLR4,Myd88,NF-κB,Muc5ac,EGFR/p-EGFR,PI3K/p-PI3K,Akt/p-AKT were detected by Western-blot,and the expression of Muc5ac was detected by immunohistochemistry.Results Bufei Huoxue Capsule could improve FVC,FEV0.3,FEV0.3/FVC and FEF25-75 in rats with chronic obstructive pulmonary disease(P<0.05,P<0.01)reduce the contents of IL-1β,IL-6,NE,TGF-β,EGF,IL-13 and TNF-α significantly(P<0.05,P<0.01);the protein expressions of TLR4,MyD88,ERK1/2,P38MAPK,NF-κB,Muc5ac,p-EGFR,p-PI3K and p-AKT were decreased.(P<0.05,P<0.01).Conclusion Bufei Huoxue Capsule can improve lung injury by inhibiting airway inflammation and mucus hypersecretion in rats with COPD.
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