The effects of Dahuang Sanwei Tablets on secretion function and structure of Intestinal mucosa and gut microbiota in constipated rats
Objective To observe the effects of Dahuang Sanwei Tablets on secretion function and structure of intestinal mucosa and gut microbiota in constipated rats. Methods 40 SD rats were randomly divided into control group (group A),model control group (group B),Dahuang Sanwei Tablets group 7 days group (group C),Dahuang Sanwei Tablets 14 days group (group D) and Dahuang Sanwei Tablets group 30 days group (group E),with 8 rats in each group. Rats in group B,C,D,E were given loperamide hydrochloride 8 mg/kg by Gavage for 7 days. After successful modelling,rats in group C,D,E were treated with Dahuang Sanwei Tablets suspension 243 mg/(kg·d)for 7 days,14 days and 30 days respectively. The curative effect of Dahuang Sanwei Tablets were evaluated by comparing the fecal water content of rats and changes of intestinal secretory function among group A,B,C and the changes of intestinal secretory function were analyzed by Masson-Fontana melanin staining method,the melanosis of colon was observed in rats. 16S rRNA high-throughput sequencing was used to analyze the changes of intestinal flora among group A,B,C and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine the changes of differential metabolites. Results Compared with group A,the fecal water content and colonic mucosal short-circuit current in group B were decreased significantly(P<0.05);compared with group B,the fecal water content and colonic mucosal short-circuit current of rats in group C were increased significantly(P<0.05).No specific melanin pigmentation and argyrophil granules were found in the colon of constipation rats after 7,14 and 30 days of Dahuang Sanwei Tablets intervention. Compared with group B,the abundance of Oscillospiraceae,Prevotellaceae,Lachnospiraceae and Muribaculaceae was increased in group C.Compared with group A,Ecabet,a metabolite of intestinal flora was significantly decreased in group B (P<0.01). Compared with group B,the content of Cinnamic Acid,the metabolite of intestinal flora,was significantly increased in group C(P<0.05).Conclusion Dahuang Sanwei Tablets can improve the structure of intestinal microflora and the change of differential metabolite by regulating the secretion function of intestinal tract in rats,and increase the water content of feces in rats with constipation with safety and effectiveness.
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